Parvin Shariati Gazgazareh; ALI AKBAR MASOUDI; Rasoul Vaez Torshizi; Alireza Ehsani; Zaynab Mousavian
Volume 22, Issue 2 , June 2020, , Pages 199-209
Abstract
The aim of this study was the investigation of gene expression profile in Shal sheep ovarian tissue using RNA sequencing data. For this purpose, the ovaries of five Shal sheep were isolated after estrous synchronization and their RNA was sequenced using Illumina Hiseq 4000 technology. On average, ...
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The aim of this study was the investigation of gene expression profile in Shal sheep ovarian tissue using RNA sequencing data. For this purpose, the ovaries of five Shal sheep were isolated after estrous synchronization and their RNA was sequenced using Illumina Hiseq 4000 technology. On average, the data obtained from the sequencing consisted of 26638311 read pairs with 81.08 unique mapping rate. The results of bioinformatic analyzes revealed the expression of 21085 genes in Shal sheep ovarian tissue, of which 15078 genes had expression mean above 10. Gene ontology analysis revealed the significant enrichment of 162 GO terms including 41 biological processes, 46 molecular functions and 75 cellular components. KEGG pathway analysis also identified 149 significant pathways (P <0.05), most important of which were estrogen signaling pathway, TGF-beta signaling pathway and oocyte meiosis. Investigating the expression of major genes for twining and reproduction, showed a high expression for INHA, INHBA and BMPR1B, so that INHA, an important paracrine factor in ovarian follicles, was one of the 10 genes with the highest expression. Also, FSHR, ESR1 and ESR2 showed medium expression and GDF9, BMP15 and PRLR showed low expression in the samples. For the first time, in this study the ovarian tissue transcriptome of Shal ewes was comprehensively studied using RNA-Seq technology and this study can provide a useful genetic basis for a better understanding of the genes and processes involved in the Shal sheep reproduction.
Rasoul Vaez Torshizi
Volume 19, Issue 2 , August 2017, , Pages 265-280
Abstract
The aim of this study was to compare the performance of immune system in Arian, Native, Arian × native crosses, native × Arian crosses and Ross chickens. Through artificial insemination, a total number of 298 birds produced and reared with 100 Ross strain chickens. Thereafter, 15 to 20 percent ...
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The aim of this study was to compare the performance of immune system in Arian, Native, Arian × native crosses, native × Arian crosses and Ross chickens. Through artificial insemination, a total number of 298 birds produced and reared with 100 Ross strain chickens. Thereafter, 15 to 20 percent of chickens were randomly chosen for immune system performance and some of blood parameters comparison. The studied traits were humoral, cell-mediated and innate immunity responses, plasma protein concentrations and relative weight of lymph organs. Data were analyzed using GLM procedure and means were compared using Tukey method. The highest titer of total antibody was in Arian strain and the lowest was in Ross strain. There was no significant difference between sexes and strains for cell-mediated immunity. Responses in crossbred chickens for humoral and cell-mediated immunity were not significantly different from Ross strain. No significant difference was observed for hematocrit percent between crossbred chickens and commercial strains. In crossbred chickens, the percentage of plasma albumin was significantly higher than the other strains (P<0.05). While, the lysozyme activity of crossbred chickens was significantly lower than Arian strain (p<0.05), it did not indicate any difference with Ross strain. The relative weight of all organs in crossbred chickens was lower than native and higher than commercial chickens. The results of current study indicated that the performance of immune system in crossbred chickens was favorable and in some cases better than the other strains. Therefore, it is possible to create resistance birds with suitable growth performance from crosses of native and commercial strains.
Hossein Emrani; Rasoult Vaes Torshizi
Volume 19, Issue 1 , May 2017, , Pages 33-45
Abstract
Ascites Syndrome is a costly metabolic disorder that has been linked to intense selection on growth rate and low feed conversion ratio in broilers. This Syndrome has become one of the most frequent causes of mortality within the broiler industry and leads to substantial economic losses and reduced animal ...
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Ascites Syndrome is a costly metabolic disorder that has been linked to intense selection on growth rate and low feed conversion ratio in broilers. This Syndrome has become one of the most frequent causes of mortality within the broiler industry and leads to substantial economic losses and reduced animal welfare. To identify genes and genomic regions associated with Ascites Syndrome, genome wide association study was performed by the chicken 60K SNP panel on 101 birds from a F2 chicken population derived from the reciprocal cross between Arian broiler Line and Azerbaijan native fowls. Genomic best linear unbiased prediction procedure was used to calculate associations between each SNP and ascite related traits. Several SNPsfor the ratio of right ventricular to total ventricular, an indicator for Ascites syndrome, reached the suggestive threshold (8.25×10-5) on chromosome 7. Two SNPs detected within CCDC141 and OSBPL6 genes were previously reported for human heart disorder. There are a large number of genes in this region (12745561-14602723) on chicken chromosome 7 that are related to hypertrophic cardiomyopathy, heart rate and human heart disorder. Selection for ascites resistance in broiler chicken using these findings could accelerate the genetic progress.
sajad pezeshki najafabadi; abolfazl shirazi
Volume 18, Issue 4 , December 2016, , Pages 679-686
Abstract
Transcription factors have important role in controls of animal fertility by binding to promoter regions of genes and regulation of their expression. In this study, nobox, ovol1 and zp3 genes expression, that are candidate genes involved in regulatory pathways of genes, in two categories of uniparous ...
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Transcription factors have important role in controls of animal fertility by binding to promoter regions of genes and regulation of their expression. In this study, nobox, ovol1 and zp3 genes expression, that are candidate genes involved in regulatory pathways of genes, in two categories of uniparous and multiparous Shall sheep were investigated. According to the pedigree of animals, a totally of six ewes selected from the herd of Qazvin Shall breeding center and applied for this experiment. After estrus synchronization, ewes were operated by general anesthesia technique and ovarian mature follicles were extracted by aspiration. After RNA extraction, sample’s cDNA was constructed. To investigation the expression of the above genes, a set of specific primers was designed for a gene, and then relative expression of these genes were measured using Real-Time PCR method and by employment of ∆∆Ct approach. Comparisons between the averages of data were done by T-Test Statistical procedure. In addition, promoter region and transcription factors of the genes investigated using BDGP software. The expression of nobox and ovol1 genes were significant in multiparous animals category (P
ali javanrouh aliabad; Ali akbar masoudi; alireza ehsani
Volume 18, Issue 4 , December 2016, , Pages 697-709
Abstract
In order to identify loci and genes associated with meat quality traits, genome-wide association study (GWAS) were conducted in a F2 population derived from a reciprocal cross between Azerbaijan native chickens and Aryan broiler line by using Illumnia 60 K Chicken SNP Bead chip. For each bird, a total ...
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In order to identify loci and genes associated with meat quality traits, genome-wide association study (GWAS) were conducted in a F2 population derived from a reciprocal cross between Azerbaijan native chickens and Aryan broiler line by using Illumnia 60 K Chicken SNP Bead chip. For each bird, a total 6 traits including water holding capacity, meat color lightness (L*), redness (a*), yellowness (b*), shear force and ultimate pH were measured. The SNPs that were associated with meat quality traits were identified using both GLM and compressed mixed linear models (CMLM). A total of 36 SNPs were associated with meat quality traits in the genome–wide significance and suggestive levels, that 3 SNPs were significantly associated with meat color yellowness through CMLM model and 18 SNPs were suggestively associated with meat color yellowness, ultimate pH, water holding capacity and shear force through GLM model. The identified candidate genes have molecular functions related to meat quality traits. So, these candidate genes can be applied in the chicken breeding scheme.
Afrooz Kamali Sangani; Ali Akbar Masoudi; Rasoul Vaez-Torshizi; Morteza Sharifi nia; Alireza Eyvazi; Majid Farahi; Behzad Rajabi Marand
Volume 18, Issue 3 , October 2016, , Pages 583-591
Abstract
This study was conducted to rank the broiler farms based on buildings, installations and equipment and its effect on production factors. To determine the contribution of each factor in ranking of farms, a multi-criteriadecision analysis (SAW) was used. Data of 108 broilers were recorded in specific forms ...
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This study was conducted to rank the broiler farms based on buildings, installations and equipment and its effect on production factors. To determine the contribution of each factor in ranking of farms, a multi-criteriadecision analysis (SAW) was used. Data of 108 broilers were recorded in specific forms and the amount of investment per broiler was calculated for individual factors. To calculate the adjusted weights of parameters, 20 experts were consulted as was specified in forms. The results showed that ventilation (fans and air inlets), roof and wall insulation in a poultry houses have represented about 55 percent of technological factors. The breeding units holding rank 1 and 2 enjoyed high degree of mechanization. As the level of mechanization was improved, the density of chickens per unit area was also increased; These units used more tunnel ventilation and large fans, and their ceiling were insulated using fiberglass, polystyrene, corrugated plastic and thickness of walls were also 35 cm. Mostly external heaters have been installed. Feeding and drinker systems have exerted no important effect on production. Slaughter weight and survival percent were not affected by the degree of mechanization but feed intake and feed conversion ratio decreased and production index increased (p<0.01) in the breeding units holding rank 1 and 2. In general in poultry house, ventilation system and ceiling and wall insulation improved energy efficiency and increased the efficiency of the system.
Sana Farhadi; Ali Akbar Masoudi; Rasoul Vaez Torshizi
Volume 17, Issue 1 , April 2015, , Pages 9-18
Abstract
The TLR4 gene structure as the main receptor for lipopolysaccharide recognition of Gram-negative bacteria was investigated in two strains of Iranian commercial Arian line and west Azerbaijan native chicks and its expression was studied in some major organs. Blood samples of the 120 birds were taken and ...
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The TLR4 gene structure as the main receptor for lipopolysaccharide recognition of Gram-negative bacteria was investigated in two strains of Iranian commercial Arian line and west Azerbaijan native chicks and its expression was studied in some major organs. Blood samples of the 120 birds were taken and total DNAs extracted. Then, the target gene was sequenced using four pairs of primers on four samples of each strain. The effects of amino acid changes on protein function were assessed by PANTHER software. To investigate the gene expression, total RNAs were extracted from liver, spleen, and lung tissues after slaughter of the birds. Gene expression was assessed by semi-quantitative RT-PCR. The amplified RNAs from tissues of liver, spleen and lungs. Then electrophoresis images were processed with the Image software and quantitative data analyzed by MINITAB. The results showed three new single polymorphisms (T1147C, C2246A and A1832G) in the sequence of TLR4 gene in the studied populations. The effects of variations on TLR4 protein structure indicated a deleterious effect of mutations on protein structure. The TLR4 gene expression in case of native and commercial strains did not show significant differences. Due to importance of the TLR4 in innate immunity and identification of some novel mutations affecting on protein structure, this gene could be a valuable candidate related to genetic resistance in poultry.
Hassan Shirzadi; Farid Fhariatmadari; Mohammad Amir Karimi-Torshizi; Shaban Rahimi; Ali Akbar Masoudi
Volume 17, Issue 1 , April 2015, , Pages 151-160
Abstract
A study was carried out to evaluate the effects of R. coriaria L. and P. farcta extracts as alternatives to antibiotic growth promoters in broilers diet. A total of 300 one-day-old male Ross 308 chicks were randomly allocated to four dietary treatments (five pen replicates, 15 birds per pen) for the ...
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A study was carried out to evaluate the effects of R. coriaria L. and P. farcta extracts as alternatives to antibiotic growth promoters in broilers diet. A total of 300 one-day-old male Ross 308 chicks were randomly allocated to four dietary treatments (five pen replicates, 15 birds per pen) for the 0- to 28-d period, and all data were analyzed in a randomized complete design. Dietary treatments included a basal diet (as control diet, without additive) and three similar diets that were supplemented with 200 mg/kg R. coriaria L. extract, P. farcta extract, and oxytetracycline 20 percent, respectively. The results showed that average daily gain was significantly increased (P<0.05) by supplementing diets with all feed additives in 7-14 d period and entire (0-28 d). Broilers fed the diets supplemented with each of extracts had a low serum cholesterol levels as compared to control diet (P<0.05). Moreover, the antibody titer against Newcastle disease virus was significantly increased by supplemented diets (P<0.05). However, adding feed additive to diets had no significant effect on antibody titer against SRBC, skin thickness due to DNCB challenge and toe-web thickness in response to injection of phytohemagglutinin-P. In addition, supplementing diet with P. farcta caused to a significant increase in relative bursa of fabricius weight in contrast with control diet. Based on current results, it can be concluded that R. coriaria and P. farcta extracts can be used as alternatives to oxytetracycline as a growth promoter in broilers diet.